As promised, here's the UoR build with -100% Districts(bugged atm, source is either Urbanist, Free Haven or they didn't cap it to 100%) and -100% Pops. Systems and Colonies are 75% respectively with Colony Ascension effectively putting Colonies at 93.125%. When it comes to -100% Pop builds, there's only so many ways you can play. Altogether the highest you can get in non-SG setups for typical Bio Empires is -120% Pops and that requires UoR -> Harmonized Society, taking Cloning Ascension while reforming to Beacon of Liberty Civic, ramming through Balance in the Middle and shifting to Fanatic Pacifist after the Civil War while also taking Harmony + Domination Traditions. That leaves basically three things you can cut. 1) UoR origin. If you opt to not roll UoR, then you can also sacrifice either Balance in the Middle or Domination Tradition and still hit -100%. You can't hit -100% Districts though because Beacon of Liberty requires Egalitarian(no getting around it). 2) Not having Fanatic Pacifist(above build is proof of that), which permits -100% Districts with Xenophile shift or 3) Not rolling Cloning Path(Purity, Mutation or Cybernetic are valid alternatives with UoR origin).
Although it doesn't permit -100% Districts, this last one I might try with a Mutation -> Purity -> Purity setup. Tier 3 flexible is hot garbage but the 2nd Purity Empire Modifier makes up for it since it yields -4 Negative Leader Traits with Talented + Erudite + Harmony(hence no need for Aptitude).
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